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Nature 584 , 120124 (2020)

Even though animal models of alcohol liquid diet indicate that nutritional deficiency has little to do with ethanol-induced neurodegeneration (Crews and Nixon, 2009), human studies report the opposite, where numerous neurological and psychiatric manifestations in alcohol dependent patients were associated with chronic malnutrition (Arts et al., 2017)

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The resulting supernatant was centrifuged at high-speed at 11,000 g for 15 min (2C) to mitochondria precipitate, after which it was washed again