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Description
For intracellular cytokine and MMP-9 detection, PC-3, DU-145, LNCaP, BPH, and TNF (10 ng/mL) pre-activated HUVECs (3 10 5 cells/per FACS tube) were fixed and permeabilized, using the CytoFix/Cytoperm kit (Becton Dickinson), and stained with the following PE-conjugated Mabs: anti-human VEGF (Clone #23410, R&D Systems), CXCL12/SDF-1 (Clone #79018, R&D Systems)

Following administration of LC, these effects were ameliorated

Long Y, Wang Y, Shen Y, Huang J, Li Y, Wu R, et al

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