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Testis protein extraction was performed using T-PER Tissue protein extraction buffer (Thermo Scientific, Waltham, MA, United States) and Halt Protease and Phosphate inhibitor cocktail (Thermo Scientific) following the manufacturers recommendation

Ca 2+ channels couple spiking to mitochondrial metabolism in substantia nigra dopaminergic neurons

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However, therapeutic strategies discussed in the available literature and reviewed here predominantly focus on managing dyslipidemia, with limited attention given to alternative approaches targeting immune dysfunction and inflammation directly