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Briefly, specimens were incubated at 4 C for 48 h with primary antibodies at a 1:200 dilution: rabbit polyclonal anti-Myosin VIIa (#25-6790, Proteus Biosciences, US), Phalloidin (#CA1640 and #CA1670, Solarbio, China), mouse monoclonal anti-SIRT1 (#Ab110304, Abcam, UK), mouse monoclonal anti-3-NT (#N5538, Sigma-Aldrich, US), rabbit polyclonal anti-4-HNE (#46,545, Abcam, UK), mouse monoclonal anti-NF (#GB12144, Servicebo, China), and rabbit anti-Phospho-AMPK1 antibody (#AP0871, Abclonal, China)

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(3) Organ-centric reductionism: the current investigations predominantly focus on isolated organ systems, neglecting BBRs pleiotropic effects within inter-organ communication networks critical to systemic metabolic regulation

Fatigue associated with autoimmune disease has a complex pathogenesis, involving inflammation, autoimmunity and neuroendocrine aspects