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Epigenetic silencing of dual oxidase 1 by promoter hypermethylation in human hepatocellular carcinoma

Substitutions of Tyr-274 by glutamate, cysteine or histidine remain enzymatically active insofar as cleaving the duplex DNA, but religation does not occur since the 3'-phosphodiester bond doesn't form, it requires the OH of Tyr-274

Principle of GST-pull down GST is fused to the tagged protein and the fused protein can be expressed in many expression systems

Infection and bleeding may occur at the injection site, but this can be avoided by cleaning the site before injection and applying pressure to the site after injection