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Shi CJ, Li SY, Shen CH, et al

Superoxide-mediated early oxidation and activation of ASK1 are important for initiating methylglyoxal-induced apoptosis process

Prior to the assay, the F12K medium was changed to unbuffered Base Medium (Seahorse Bioscience) supplemented with 2 mM glutamine (Invitrogen), 2 mM pyruvate (Sigma), and 7.1 mM glucose (Sigma) adjusted to at pH 7.4 with NaOH for 1 h at 37 C

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