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vivo Ann Rheum Dis (2004) 63:8846
Immunofluorescence (IF) microscopy Fission yeast cells (1 10 8 cells at OD 595 = 0.5) were fixed with 3% paraformaldehyde (pFA) at 26 C for 10 min, and fixation was quenched by incubation with glycine (final 0.25 M) for 5 min
Lectins are drawn to a polysaccharide, glycoprotein, or cell surface receptor, while nucleic acids are drawn to their precise complementary base sequence, histone, nucleic acid polymerase, or nucleic acid binding protein
