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Once NAC is inside the cells it is rapidly hydrolyzed, then cytosolic acylase I deacetylates NAC ( Figure 2Antioxidant activity of NAC.Increasing reactive oxygen species (ROS) depletes the antioxidant enzymes in the cells

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Morgan et al., Macrophage-mediated GDNF delivery protects against dopaminergic neurodegeneration: a therapeutic strategy for Parkinsons disease

The reaction was initiated by adding 10 L of 5 mM ATP and incubated at 37 C for 30 min