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(D) Lamin line (arrowhead) in a D2 nucleus stained for nuclear pores (green, NPP-9/RanBP2)

The process was fast and easy

We therefore sought to benchmark our approach by assessing MV dimensions resolved on ExM in metabolically labelled larvae against those visualized with transmission electron microscopy (TEM), the gold standard for morphological studies of ultrastructures in nematodes (Figure 4C-F)