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Cells were fixed with 4% PFA for 10 min at room temperature (RT) and permeabilized with 0.15% Triton X-100 in PBS for 15 min

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Results indicated that 279 natural compounds (62 terpenoids, 57 alkaloids, 52 flavonoids, 26 phenols, 19 phenylpropanoids, 9 steroids, 9 saponins, 8 quinonoids, 6 vitamins, and 31 others) can act on various mechanisms to improve UC, such as regulating gut microbiota and metabolites (Mechanisms I), protecting the intestinal mucosal barrier (Mechanisms II), regulating intestinal mucosal immune response (Mechanisms III), as well as the other mechanisms (cellular autophagy modulation and ferroptosis inhibition)

, 267 , 6102-6109, Schomburg, L., and Khrle, J