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Culture medium was replaced with a isotonic bath solution for 1 h and cells were exposed to isotonic solutions (PBS-like containing 138 mM NaCl, 2.7 mM KCl, 1.4 mM KH 2 PO 4 , 8 mM Na 2 HPO 4 , 10 mM HEPES, 2 mM MgCl 2 , 0.4 mM CaCl 2 , Pos ~300 mOsm.l 1 ), hypotonic solutions (46 mM NaCl, 2.7 mM KCl, 0.5 mM KH 2 PO 4 , 3 mM Na 2 HPO 4 , 10 mM HEPES, 2 mM MgCl 2 , 0.4 mM CaCl 2 , Pos ~100 mOsm.l 1 ) or isotonic solutions containing MSU or CPP crystals (200 g/ml) in the presence or absence of different inhibitors

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NAC was observed to have an in vitro antimicrobial effect against all 38 bacterial isolates tested at relatively low concentrations (0.1560.625%, 1.566.25 mg/mL) [136]

894 Alternatively, the construction of large liposomes enables the encapsulation of whole cells and other components to form hybrid cells capable of layered functionalities