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Description
DTNB (Ellman's Reagent) is a chemical used for quantifying the amount or concentration of sulfhydryl groups

Sometimes it delivers nothing at all

It shows in Domain-1 the thioredoxin canonical folding, with the three parallel beta-sheets (124) and one antiparallel (3)

3a) and involves negatively charged amino acids on four different helices (1, 2, 5 and 9) of the SemDs rigid core (Fig