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First, although we initially planned to include five mice per group, one animal in the tirzepatide-treated group died just before drug administration (day 0), and an additional mouse died on day 10 after treatment initiation
The finding of Ileibacterium as the most abundant cecal microbiome genus in every experimental group is relatively novel and may reflect experimental factors such as diet, housing conditions, and mouse strain
Researchers commonly study one single-agonist and one multi-agonist side-by-side in the same experiment
