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introduction of two random mutations of Y444A and G484A to D433N resulted in up to 50-fold increase in catalytic efficiency for GL-7-ACA substrate (Yamada et al., 2008)
The peptide Herdegradin, which downregulates EGFR, stimulates mitophagy through the mTORC2/Akt pathway, effectively inhibiting the growth of EGFR-positive cancers, such as PCa, ultimately inducing the death of PCa cells (Katreddy et al., 2018)
After dialysis with Tris pH 8, the protein was purified and concentrated to 22 mg ml 1 (final buffer 50 mM Tris pH 9, 150 mM NaCl and 10% glycerol)
These investigations often rely on research-grade peptides as tools for controlled experimental analysis
