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Antioxidants (Basel) 11 (12), 2345 (2022)

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Sections were incubated overnight at 4C with primary antibodies against CD68 (1:2000, Proteintech, Wuhan, China), NOS2 (1:400, ABclonal, Wuhan, China), HO-1 (1:400, ABclonal, Wuhan, China), SOD2 (1:400, ABclonal, Wuhan, China) and nuclear factor E2-related factor 2 (NRF2) (1:200, Proteintech, Wuhan, China), and cells were incubated with primary antibodies against NRF2 and NF-B P65 (1:400, Cell Signaling Technology, Danvers, USA), followed by incubation with fluorophore-conjugated secondary antibodies