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Quantitative PCR was performed using Universal SYBR Green Supermix (Biorad, Hercules, CA) and run on a Viia7 Real-Time PCR system (ThermoFisher Scientific)
The first air curtain has a first temperature and the second air curtain has a temperature lower than the first temperature
Nitric oxide Nitric oxide (NO) is a hydrophobic gasotransmitter that readily diffuses through biological membranes and plays a pivotal role in retrograde signaling during CNS development and physiological processes [155, 156]
Expert Rev Clin Immunol 7(3):287294
