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The absorption coefficient of proteins is often expressed as E 1% (10 mg/mL solution)

For longer-term storage, divide into sterile aliquots and store at -20C

For sandwich ELISA, Maxisorp plates were coated overnight at 4 C with a polyclonal goat anti-HSA antibody (Sigma, # A-7544) diluted 1:1000 in PBS for the unfused HSAs or with monoclonal rabbit anti-camelid VHH antibody (Genscript, # A01860) diluted 1:1000 in PBS for the Albu-LiTE fusion proteins

Cluster 2 (T2, T5-T6, T10, T14) exhibits a distinct phytochemical profile, characterized by: (i) higher phenolic acid content relative to other clusters, with p -coumaric acid being particularly abundant, and (ii) reduced levels of all curcuminoids except curcumin I