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After 5 seconds at room temperature, the relative light unit (RLU) values were measured using a luminometer (Model 680, Bio-Rad, Hercules, CA, USA)

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To address these, advanced synthesis techniques, like solgel methods and plasma-enhanced chemical vapor deposition (PECVD), ensure uniform nanoparticle dispersion, while surface modifications with silica or polymeric layers enhance durability and minimize environmental impact

These changes manifest, in part, as alterations in gene and protein expression that contribute to retinal inflammation, microvascular defects, and neuronal dysfunction [192]