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High performance liquid chromatography was used to change the salt, the main peak of the salt exchange was collected and the purity was detected by analyzing the liquid phase, and the solution of the main peak of the salt exchange was combined with the solution of the main peak of salt exchange, and the aqueous solution of canagliptide acetate was obtained by freeze-drying, and the purity was 99.58%, the maximum single impurity was 0.09%, the total yield was 31.87%, and the molecular weight was 4409.0

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For compound treatment, the medium was replaced by 90% of Williams E Medium supplemented with 12.5% HepaRG preinduction and toxicity medium supplement, 0.6% HepaRG induction medium supplement (Lonza, catalog: MHIND), and 25 U/mL penicillin-25 g/mL streptomycin