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Unless otherwise specified, the cells were treated as follows: For EBSS treatment, cells were cultured in EBSS (Gibco, 24010043) for 6 h after being washed three times with PBS (Sangon Biotech, E607008)

Since the use of growth hormone in IVF is considered by the FDA as an off-label use of a medication without long-term studies of safety, RSC is hesitant to add this expensive injection without knowing there is a clear problem with egg quality

tricolor under drought stress that may have related to satisfactory regulation of H 2 O 2 in the tolerant variety VA13

These steps were performed using air-saturated buffers