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Lipoperoxides were determined by the addition of 200 l of the supernatant to 800 l of a reaction mixture containing 0.5% thiobarbituric acid (solubilized in 20% TCA) and an incubation at 100C for 30 min

Mol Cell Proteomics (2016) 15(11):341223.10.1074/mcp.M116.060426 90 GidrewiczDAFentonTR

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Native reversed-phase liquid chromatography: a technique for lcms of intact antibodydrug conjugates
