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Biochemical AMPylation assays One microgram of His 6 -Ceg14 or its mutants, 1-g actin (Cytoskeleton, cat# APHL99) and 5 g His 6 -Profilin were incubated in a 20 L reaction system containing 50 mM Tris-HCl (pH 7.5), 5 mM MgCl 2 , 1 mM DTT and 100 M biotin-17-ATP for 2 h at 37 C
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This shift resulted from enhanced recycling of GSSG back to GSH via upregulated glutathione reductase activity
Toward a Genome Scale Sequence Specific Dynamic Model of Cell-Free Protein Synthesis in Escherichia Coli
