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Specifically, ARPE19 cells (150 cm 2 ) were cultured as described above until 80% confluency, harvested, washed with PBS, and resuspended in 7 mL of homogenization buffer (20 mM HEPES, pH 7, 0.1 M KCl, protease inhibitors)

For example, a 10 mg vial reconstituted with 200 units of water gives 0.05 mg per unit

Using RT, we were able to monitor the dynamic changes of GSH in living cells, which subsequently led to the observation of enhanced antioxidant capability of activated neurons and time-dependent changes of GSH during the ferroptosis process

GGT1 drives GSH catabolism and survival Several enzymes have GGT activity 38 , and GGT1 is the most catalytically active isoform 39