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Description
Both ramp and accumulation times were set to 100 ms
Cell pellets were resuspended in reducing lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 10 mM DTT, 0.01 mg ml 1 DNase I) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator
A meta-analysis of these studies found no significant difference in LLT between the thermal pulsation system (LipiFlow) and other treatments, including placebo, warm compresses, or alternative thermostatic devices
Introduction to the moringa family: Origin, distribution and biodiversity
