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0.05 g of acridine orange was diluted with distilled water to 100 mL to prepare a 0.5 mg/mL acridine orange stock solution, which was then diluted 1:100 to a final concentration of 5 g/mL

Further study is needed to characterize adduct profiles in patients receiving low doses of APAP with preexisting disease (e.g., liver disease) to better understand the potential application of this biomarker across a range of APAP exposures