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Glut4 palmitoylation at Cys223 plays a critical role in Glut4 membrane trafficking
Reconstituted solutions should be protected from reducing agents (such as DTT or beta-mercaptoethanol), which can cleave the disulfide bond and disrupt the active conformation
Kraus et al., Nature Medicine highlight that sustained NNMT inhibition drives metabolic changes, but strategic breaks may help preserve efficacy over time
