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Characterization of microglial reaction after middle cerebral artery occlusion in rat brain

Chemical trapping experiments of 16 O 2 ( 1 g ) and 18 O 2 ( 1 g ) were performed using the anthracene-9,10-diyldiethane-2,1-diyl disulfate disodium salt (EAS) trap by monitoring the corresponding endoperoxide (EAS x O x O, x = 16 or 18) with high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS)

Many patients notice relief within the first 2-3 sessions
