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Description
LCMS/MS analysis was performed using an Advance nanoLC (BrukerMichrom, Auburn, CA, USA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific) equipped with a NANOHPLC capillary column C18 (0.075 mm ID 150 mm length, 3 m particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 535% CH 3 CN /0.1% formic acid) at a flow rate of 300 nL/min
[DOI] [PMC free article] [PubMed] [Google Scholar] 293.Geng T., Li P., Okutsu M., et al

doi: 10.1038/ncomms4592 23 LevyH

Insulin-like growth factor-binding proteins in serum and other biological fluids: regulation and functions
