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You might look at some books by Dr

(2005) cloned and characterized five GS-like proteins from soybean, wheat, and corn noting that the soybean enzyme was an hGS and that all the other enzymes functioned as GS

Then, the strips were equilibrated in equilibration buffer containing 2% (w/v) SDS, 1% (w/v) DTT, 50 mmol/L Tris-HCl, pH 8.8, 20% (v/v) glycerol, 6 mol/L urea, 0.002% (w/v) bromophenol blue and 1.5% (w/v) iodoacetamide for 15 min at room temperature

Anterior horn cells are a type of motoneuron emerging from the anterior portion of the gray matter in the spinal cord toward the skeletal muscle, while alpha motoneurons () are the lower motoneurons found in the brainstem and spinal cord and are the largest neurons in the spinal cord with myelinated axons