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The mitochondrial suspension was then centrifuged at 8500 g for 20 min at 4 C and the remaining pellet was resuspended in TRIzol reagent (15596018, Invitrogen) for downstream extraction of mitochondrial RNA using Direct-zol RNA miniprep (R2055, Zymo) with DNase treatment

In a few pathogens, including null-strains of Bordetella pertussis , Neisseria meningitidis and Haemophilus influenzae , the lipopolysaccharides lack an O-chain and are sometimes termed 'lipooligosaccharides' and tend to have more complex and highly decorated core structures that are responsible for bacterial specificity

doi: 10.1016/j.canlet.2005.10.041 40 TakamoriHOadesZGHochOCBurgerMSchraufstatterIU