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Description
Cell debris was removed by centrifugation at 10,000 rpm at 4 C and the cell lysate incubated with Ni-NTA beads (Qiagen) for 1 h at 4 C at a rotary shaker

The Role of Components of Bifidobacterium and Lactobacillus in Pathogenesis and Serologic Diagnosis of Autoimmune Thyroid Diseases

M.FaganS

10.3390/molecules190914094
