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Messenger RNA was purified using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) following manufacturers protocol, scaled up to 100 g of total RNA input per sample

Neufeld EJ, Galanello R, Viprakasit V, Aydinok Y, Piga A, Harmatz P et al (2012) A phase 2 study of the safety, tolerability, and pharmacodynamics of FBS0701, a novel oral iron chelator, in transfusional iron overload