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To evoke action potentials in LOC efferents, electrical field stimulations (30 V, 5 Hz, 0.5 ms impulse duration, for 3 min) was applied in the 3rd (S 1 ) and 13th (S 2 ) fractions by a Grass S88 stimulator (Grass Instrument Co., Quincy, MA) via platinum electrodes positioned at the upper and lower parts of the tissue chamber

The tissue analysis procedures were conducted swiftly to prevent thawing

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