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Western blotting Cells lysates were collected in 1X sample buffer (2% SDS, 10% glycerol, 0.01% bromophenol blue, 62.5mM Tris, pH 6.8, 0.1M DTT) and boiled to 95C for 10 min
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The washout refers to the time between the first three week trial and the second three week trial where the LCLT and placebo groups are switched and both groups stop taking their assigned supplements
