cysteine synthesis of glutathione Figure 2 from Regulation neuronal Pathway showing biosynthesis and metabolism
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The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C

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Vitamin B12 is found almost exclusively in animal-based foods like meat, fish, eggs, and dairy
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