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DJ-1 overexpression increased the ER-mitochondria connection and DJ-1 knockout resulted in significant functional impairment of MAMs both in vitro and in vivo (Liu et al., 2019)
for example, you would would subtract .03 for 2 hours
Intracellular ROS levels depend on the balance between ROS generation and clearance, and are also manipulated by antioxidant defense mechanisms [6]
Each of these play important roles in antioxidant defense, protein folding, and signal transduction
