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Recognition and receptor-mediated endocytosis of the lysosomal acid lipase secreted by cultured human fibroblasts

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After 5 days, detached fibroblasts were re-seeded onto mitomycin C (AG scientific, San Diego, CA, USA, #M-1108)-treated mouse embryonic fibroblasts and cultured for 14 days in reprogramming medium [DMEM/F12, 1 mM MEM Non-Essential Amino Acids Solution (NEAAs, Gibco, Thermo Fisher Scientific, #11140035), 1% P/S, 0.1 mM 2-Mercaptoethanol (Gibco, Thermo Fisher Scientific, #21985023), 20% KnockOut Serum Replacement (KSR, Gibco, Thermo Fisher Scientific, #10828028), and 20 ng/ml basic fibroblast growth factor (bFGF, R&D Systems, #233-FB, Minneapolis, MN, USA)]