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Drug self-administration can be significantly influenced by environmental conditions (e.g., feeding conditions, palatable dietary substances, stress) and individual differences (e.g., reactivity level, age, sex, dietary preferences, genetics) even under the same pharmacological conditions such as drug dose, drug history, and pretreatment drugs 69

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The proteins were transferred to nitrocellulose membrane and then blots were blocked with 5% (w/v) non-fat milk in Tris buffered saline with Tween (TBS-T) and incubated with mouse anti-GFAP antibody (1500